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KMID : 1094719980030020112
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Biotechnology and Bioprocess Engineering
1998 Volume.3 No. 2 p.112 ~ p.112
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Enhancement ofcis,cis-muconate productivity by overexpression of catechol 1,2-dioxygenase inPseudomonas putida BCM114
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Kim Beum-Jun
Choi Won-Jae
Lee Eun-Yeol
Choi Cha-Yong
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Abstract
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For enhancement ofcis,cis-muconate productivity from benzoate, catechol 1,2-dioxygenase (C12O) which catalyzes the rate-limiting step (catechol conversion tocis,cis-muconate) was cloned and expressed in recombinantPseudomonas putida BCM114. At higher benzoate concentrations (more than 15 mM),cis,cis-muconate productivity gradually decreased and unconverted catechol was accumulated up to 10 mM in the case of wildtypeP. putida BM014, whereascis,cis-muconate productivity continuously increased and catechol was completely transformed tocis,cis-muconate forP. putida BCM114. Specific C12O activity ofP. putida BCM114 was about three times higher than that ofP. putida BM014, and productivity was enhanced more than two times.
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KEYWORD
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Pseudomonas putida BCM114, cis,cis-muconate, catA cloning, catechol 1,2-dioxygenase
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